Opis: RNA Interference - David R. Engelke, D Engelke

This book offers a comprehensive guide to RNAi experimentation and provides in depth coverage of the application of this technology in plants, flies and mammalian cells. The chapters reflect recent developments in this rapidly growing field. Written in clear concise language, "RNA interference (RNAi): Nuts and Bolts of siRNA technology" serves as an ideal resource for undergraduates and experienced research scientists. -- Gary Hardiman, Ph.D., Author of "Microarrays Methods and Applications"Chapter 1 Targeted Gene Silencing in Plants Using RNA Interference; Chapter 2 RNAi in Caenorhabditis elegans; Chapter 3 Application and Analysis of RNAi in Drosophila Systems; Chapter 4 Gene Silencing by Synthetic siRNA Duplexes in Mammalian Cell Culture; Chapter 5 Strategies for Synthesizing Small Interfering RNA (siRNA); Chapter 6 Generating Long dsRNA, Individual siRNA and siRNA Cocktails In Vitro; Chapter 7 Optimizing High Throughput RNAi-based Assays Using Transient Transfection of Synthetic siRNAs in Cultured Mammalian Cells; Chapter 8 Subcellular Delivery and Detection of Small Interfering RNA; Chapter 9 Downregulation of Cellular Genes by PCR Products Expressing siRNAs Mammalian Cells; Chapter 10 Generating Adenoviruses for shRNA or siRNA Delivery; Chapter 11 Achieving Stable, Heritable Gene Silencing in the Mouse

Szczegóły: RNA Interference - David R. Engelke, D Engelke

Tytuł: RNA Interference
Autor: David R. Engelke, D Engelke
Producent: DNA Press
ISBN: 9780966402780
Rok produkcji: 2004
Ilość stron: 242
Oprawa: Twarda
Waga: 0.46 kg

Recenzje: RNA Interference - David R. Engelke, D Engelke