Opis: Plant Phosphoproteomics

This detailed volume addresses recent developments in phosphoproteomic techniques with a particular focus on the plant system. Over the recent decades, proteomic methods were refined to study the significance and dynamics of protein phosphorylation in various biological contexts. However, working with plant tissue imposes particular challenges to the biologist which are attributed to the rigid cell wall making protein extraction more difficult, the skewed protein abundance with Rubisco as a highly abundant protein and a large central vacuole leading to low protein yield and increased degradative enzyme activity. The methodologies in this book seek to move beyond these issues. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Plant Phosphoproteomics: Methods and Protocols serves as an ideal reference for researchers investigating this vital area of plant science.1. The Plant Kinome Monika Zulawski and Waltraud X. Schulze 2. Phosphatases in Plants Alois Schweighofer and Irute Meskiene 3. Phosphoproteomics in Cereals Pingfang Yang 4. Screening of Kinase Substrates Using Kinase-Knockout Mutants Taishi Umezawa 5. Phosphopeptide Profiling of Receptor Kinase Mutants Xu Na Wu and Waltraud X. Schulze 6. Combining Metabolic 15N Labelling with Improved TandemMOAC for Enhanced Probing of the Phosphoproteome Martin Thomas, Wolfgang Hoehenwarter, Uwe Conrath, and Gerold J.M. Beckers 7. Kinase Activity and Specificity Assay Using Synthetic Peptides Xu Na Wu and Waltraud X. Schulze 8. Absolute Quantitation of Protein Post-Translational Modification Isoform Zhu Yang and Ning Li 9. Phosphorylation Stoichiometry Determination in Plant Photosynthetic Membranes Bjorn Ingelsson, Rikard Fristedt, and Maria V. Turkina 10. Phosphopeptide Immuno-Affinity Enrichment to Enhance Detection of Tyrosine Phosphorylation in Plants Sharon C. Mithoe and Frank L.H. Menke 11. The Peptide Microarray ChloroPhos1.0: A Screening Tool for the Identification of Arabidopsis thaliana Chloroplast Protein Kinase Substrates Anna Schonberg and Sacha Baginsky 12. Plant Protein Kinase Substrates Identification Using Protein Microarrays Shisong Ma and Savithramma P. Dinesh-Kumar 13. Targeted Analysis of Protein Phosphorylation by 2D Electrophoresis Kristin Mayer, Sally Albrecht, and Andreas Schaller 14. Computational Phosphorylation Network Reconstruction: Methods and Resources Guangyou Duan and Dirk Walther 15. Computational Identification of Protein Kinases and Kinase-Specific Substrates in Plants Han Cheng, Yongbo Wang, Zexian Liu, and Yu Xue 16. Databases for Plant Phosphoproteomics Waltraud X. Schulze, Qiuming Yao, and Dong Xu 17. Phosphorylation Site Prediction in Plants Qiuming Yao, Waltraud X. Schulze, and Dong Xu

Szczegóły: Plant Phosphoproteomics

Tytuł: Plant Phosphoproteomics
Wydawnictwo: Humana Press
ISBN: 9781493926473
Rok wydania: 2015
Ilość stron: 231
Oprawa: Twarda
Waga: 0.68 kg

Recenzje: Plant Phosphoproteomics