Opis: Pulse Field Gel Electrophoresis

This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.Part I: Introduction to PFGE 1. Pulsed-field Gel Electrophoresis for Disease Monitoring and Control John Besser 2. Harmonization of PFGE Profile Analysis by using Bioinformatics Tools: Example of the Listeria monocytogenes European Union Reference Laboratory Network Benjamin Felix, Sophie Roussel, and Bruno Pot 3. PFGE as a Tool to Track Listeria monocytogenes in Food Processing Facilities: Case Studies Marion Dalmasso and Kieran Jordan 4. Case Study of the use of Pulsed Field Gel Electrophoresis in the Detection of a Food-borne Outbreak Niall De Lappe and Martin Cormican Part II: PFGE and Pathogenic Bacteria 5. Pulsed-Field Gel Electrophoresis for Listeria monocytogenes Laura Luque-Sastre, Seamus Fanning, and Edward M. Fox 6. Pulsed-field Gel Electrophoresis (PFGE) for Pathogenic Cronobacter Species Qiongqiong Yan and Seamus Fanning 7. Pulsed-Field Gel Electrophoresis of Bacillus cereus Group Strains Paul Drean and Edward M. Fox 8. Pulsed-Field Gel Electrophoresis of Staphylococcus aureus George R. Golding, Jennifer Campbell, Dave Spreitzer, and Linda Chui 9. The Use of Pulsed-Field Gel Electrophoresis for Genotyping of Clostridium difficile Wondwossen A. Gebreyes and Pamela R. Fry 10. Molecular Subtyping of Clostridium botulinum by Pulsed-Field Gel Electrophoresis Carolina Luquez, Lavin A. Joseph, and Susan E. Maslanka 11. Pulsed Field Gel Electrophoresis of Yersinia pestis Tamara Revazishvili and Judith A. Johnson 12. Pulsed Field Gel Electrophoresis of Group A streptococci Luca Agostino Vitali, Giovanni Gherardi, and Dezemona Petrelli 13. Application of Pulsed Field Gel Electrophoresis to Type Campylobacter jejuni Ping Zhou and Omar A. Oyarzabal 14. Pulsed-field Gel Electrophoresis of Pseudomonas aeruginosa Honghua Hu and Jim Manos 15. PFGE for Shiga toxin-producing Escherichia coli O157:H7 (STEC O157) and non-O157 STEC Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, and Efrain M. Ribot 16. Pulsed Field Gel Electrophoresis (PFGE) of Salmonella enterica Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, and Nicola Pugliese PART III: PFGE and Non-Pathogenic Bacteria 17. PFGE Protocols to Distinguish Subspecies of Lactococcus lactis Pascal Le Bourgeois, Delphine Passerini, Michele Coddeville, Maeva Guellerin, Marie-Line Daveran-Mingot, and Paul Ritzenthaler 18. The use of PFGE Method in Genotyping of Selected Bacteria Species of the Lactobacillus genus Tomasz Gosiewski and Monika Brzychczy-Wloch 19. Pulsed Field Gel Electrophoresis for Leuconostoc mesenteroides and pseudomesenteroides Victoria Chuat and Marion Dalmasso 20. Pulsed Field Gel Electrophoresis for Bifidobacterium Esther Jimenez, Marta Gomez, and Laura Moles 21. Pulsed Field Gel Electrophoresis for Dairy Propionibacteria Victoria Chuat, Rosangela de Freitas, and Marion Dalmasso

Szczegóły: Pulse Field Gel Electrophoresis

Tytuł: Pulse Field Gel Electrophoresis
Wydawnictwo: Humana Press
ISBN: 9781493925988
Rok wydania: 2015
Ilość stron: 278
Oprawa: Twarda
Waga: 0.73 kg

Recenzje: Pulse Field Gel Electrophoresis