Opis: Angiotensin Protocols - D Wang

Donna Wang and a panel of hands-on experts describe in step-by-step detail all the major molecular, biochemical, and functional techniques now used in probing the renin-angiotensin system. These range from the use of embryonic gene targeting to create of mutant strains, to the functional investigation of components of the renin-angiotensin system. Comprehensive and highly practical, "Angiotensin Protocols" translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in health and disease today. "This is an advanced treatise on the renin-angiotensin system (RAS) and its regulation of the cardiovascular, renal, and neural relationships in health and disease. The introduction is ample to move the biological scientist reader up to adequacy to comprehend a complex patient example. ...The purpose shows a goal along with suitable objectives to move traditional medicine into utilization of the amazing explosion of modern biological techniques in RAS applications. It does so with a compilation of experts that foster a clearer picture of the modem RAS field. These are indeed worthy objectives, that surpass what individual papers and review articles can accomplish. Indeed it is needed and it serves as a tremendous thinking stimulator...The introduction by John E. Hall sets the tone and makes this book happen. The genetic manipulation of the RAS by Sigmund and Stec keeps the happening alive. the quantification of angiotensin II receptors by Wu, Yao and Wang is an outstanding peace of work. The cyclic AMP and GMP by Ji was helpful as was the NO from assay work of Thorup and Moore. The closing article on cerebral circulation by Lombard carries the exciting nature of the book to the interested student."-Doody's Health Sciences Book Review Journal "This book with great attention to the recent developments in molecular genetic, biochemical and fysiological techniques can be recommended to all researchers involved in the fundemental research of the RAS at the cellular level." - Acta CardiologicaPart I. Introduction to the Renin-Angiotensin System Historical Perspective of the Renin-Angiotensin System John E. Hall Three Angiotensin Paradigms in One Patient: Etiology of Hypertension, Glomerular Hemodynamics, and Long-Term Glomerular Protection Cheryl L. Laffer and Fernando Elijovich Part II. Transgenic and Gene Targeting Methods Production and Use of Chimeric Mice Junji Takaya, Taiji Matsusaka, and Iekuni Ichikawa Genetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-Recombinase Curt D. Sigmund and David E. Stec Systemic Delivery of a Transgene in Intact Animals by a Retroviral Vector Michael J. Katovich, Hong-Wei Wang, Craig H. Gelband, and Mohan K. Raizada Antisense Inhibition of the Renin-Angiotensin System Dagmara Mohuczy and M. Ian Phillips Part III. Analysis of Transcriptional and Translational Mechanisms Analysis of Transcriptional Control Mechanisms I: Techniques for Characterization of cis-Regulatory Elements Christopher T. Sherman and Allan R. Brasier Analysis of Transcriptional Control Mechanisms II: Techniques for Characterization of trans-Acting Factors Allan R. Brasier, Christopher T. Sherman, and Mohammad Jamaluddin Analysis of Cytosolic Proteins that Bind to the 5' Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift Assay Zheng Wu, Kamakshi Krishnamurthi, Koby Mok, and Kathryn Sandberg In Vitro Translation of the Angiotensin AT1 Receptors in Wheat-Germ Extracts Hong Ji, Kamakshi Krishnamurthi, Zheng Wu, and Kathryn Sandberg Part IV. Assays for Determination of mRNA Levels Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction Wei Wu, Aqing Yao, and Donna H. Wang Analysis of RNA by Northern-Blot Hybridization Patricia E. Gallagher and Debra I. Diz Use of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAs Scott C. Supowit and Donald J. DiPette In Situ Hybridization Susan Riggs Runge, Zhaoyong Hu, and Marschall Runge Part V. Assays for Determination of Protein Contents and Enzyme Activity Immunohistochemical Detection of Angiotensin II Receptor Zhi-Qin Wang and Robert M. Carey Western Blotting Nageswara R. Madamanchi and Marschall S. Runge Quantification of Angiotensin-Converting Enzyme (ACE) Activity Qing Cheng Meng and Kathleen H. Berecek Quantification of Angiotensin Peptides Qing Cheng Meng and Kathleen H. Berecek Radiolabeling of Angiotensin Peptides Robert C. Speth and Joseph W. Harding Assays for Radiolabel-Photoaffinity Labeling of Angiotensin Receptors Jacqueline Perodin, Antony A. Boucard, Richard Leduc, Emanuel Escher, and Gaetan Guillemette Radioligand Binding Assay Gaetan Thibault and Ernesto L. Schiffrin Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues Siew Yeen Chai, Andrew M. Allen, Jialong Zhuo, Ingrid Moeller, and Frederick A. O. Mendelsohn Part VI. Biochemical Studies of the Renin-Angiotensin System Measurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells: Fluorescence Imaging of Cytosolic Calcium Rhian M. Touyz and Ernesto L. Schiffrin Measurement of Phospholipidase D Activation in Vascular Smooth Muscle Cells Rhian M. Touyz and Ernesto L. Schiffrin Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor Hong Ji Measurement of Nitric Oxide Formation Christian Thorup and Leon C. Moore Measuring Renin Secretion from Juxtaglomerular Cells Armin Kurtz and Frank Schweda Part VII. Functional Studies of the Renin-Angiotensin System Measurement of Regional Blood Flow in the Kidney Using Laser-Doppler Flowmetry Richard J. Roman, David L. Mattson, and Allen W. Cowley, Jr Measurement of Renal Tubular Angiotensin II John D. Imig and L. Gabrial Navar The In Vitro Blood-Perfused Juxtamedullary Nephron Technique Edward W. Inscho In Vitro Proximal Tubule Perfusion Preparation Jeffrey L. Garvin and Peter J. Harris Isolated Resistance Artery Preparation Jos P. M. Wesselman, Paul H. Ratz, and Russell L. Prewitt Measurement of Vascular Density Andrew S. Greene and Mark J. Rieder Functional Studies of the Cerebral Circulation Julian H. Lombard Index

Szczegóły: Angiotensin Protocols - D Wang

Tytuł: Angiotensin Protocols
Autor: D Wang
Producent: Humana Press
ISBN: 9780896037632
Rok produkcji: 2000
Ilość stron: 544
Oprawa: Twarda

Recenzje: Angiotensin Protocols - D Wang