Opis: Transcriptional Regulation in Eukaryotes - Stephen T. Smale, Michael Carey, M Carey

In the genome era, the analysis of gene expression has become a critical requirement in many laboratories. But there has been no comprehensive source of strategic, conceptual, and technical information to guide this often complex task. "Transcriptional Regulation in Eukaryotes" aims to answer that need. Written by two experienced investigators, Michael Carey and Stephen Smale at the UCLA School of Medicine, and based in part on the Gene Expression course taught at Cold Spring Harbor Laboratory, this book directly addresses all the concerns of a laboratory studying the regulation of a newly isolated gene and the biochemistry of a new transcription factor. Chapter 1 reviews the state-of-the-art in the RNA polymerase II transcription field; while chapters 2 to 10 are a systematic guide to the investigation of regulatory mechanisms for newly identified genes. Chapters 11 to 15 discuss approaches for synthesizing recombinant transcription factors, analyzing their binding, and deciphering their mode of action. This text reveals not only what to do but why, and how to plan properly for success. This approach should be useful for graduate students, physician-scientists, postdocs, and others entering the field, but is also valuable for established investigators undertaking in-depth studies in specific systems. Supplementing clearly written text with extensive illustrations, this text puts methods in the context of underlying theory and offers expert recommendations on experimental strategies, and encourages creativity in investigative design. Protocols for essential techniques are presented in a step by step format, together with extensive advice on troubleshooting.A primer on transcriptional regulation in mammalian cells; initial strategic issues; modes of regulating mRNA abundance; transcription initiation site mapping; functional assays for promoter analysis; identification and analysis of distant control regions; identifying cis-acting DNA elements within a control region; identification of DNA-binding proteins and isolation of their genes; confirming the functional importance of a protein-DNA interaction; in vivo analysis of an endogenous control region; approaches for the synthesis of recombinant transcription factors; identifying and characterizing transcription factor domains; theory, characterization and modelling of DNA binding by regulatory transcription factors; crude and fractionated systems for in vitro transcription; approaches for studying transcription complex assembly.

Szczegóły: Transcriptional Regulation in Eukaryotes - Stephen T. Smale, Michael Carey, M Carey

Tytuł: Transcriptional Regulation in Eukaryotes
Autor: Stephen T. Smale, Michael Carey, M Carey
Producent: Cold Spring Harbor Laboratory Press
ISBN: 9780879696351
Rok produkcji: 2001
Ilość stron: 640
Oprawa: Miękka
Waga: 1.85 kg

Recenzje: Transcriptional Regulation in Eukaryotes - Stephen T. Smale, Michael Carey, M Carey