Opis: Cap-Analysis Gene Expression - P Carninci

The output of eukaryotic genomes is much more complex than expected. Genes produce different variants of RNAs from multiple promoters. One of the ultimate targets of biological analysis is to establish a relationship between the messenger RNAs that are transcribed from the genome and the genomic regions that control their expression - the promoters - in order to decipher the networks that regulate gene expression and the transcription factors that act as master regulators of transcriptional control. Novel technologies have recently appeared that allow deciphering of transcriptional network, based on the identification of the starting site of gene transcription, with the simultaneous measurement of expression level and identification of the promoter elements.These tagging technologies (including cap-analysis gene expression - CAGE - and others) are further boosted from the development of the novel generation of sequencing instruments, which allow transcriptional profiling by sequencing at the cost of microarray experiments. This book is a guide for users of new technologies, as it includes accurately proven protocols, allowing readers to prepare their samples for experiments. Additionally, it provides a guide for the bioinformatics tools that are available for the analysis of the obtained tags, including the design of the software, the sources and web information where they can be downloaded. Finally, the book provides examples of the application of these technologies to identify promoters, annotate genomes, identify new RNAs and reconstruct models of transcriptional control. Although examples mainly regard mammalians, the discussion expands to other groups of eukaryotes, where these approaches are complementing genome sequencing. "This is a comprehensive introduction to a powerful and important new technique that is transforming our understanding of gene regulation. This volume contains everything you need to know to become an expert in CAGE, from library construction to bioinformatic analysis of the voluminous data sets." --Gene E. Robinson, University of IllinoisBrief Historical Perspective on Transcriptional Regulation. Preparation of CAGE Libraries: An Enabling Experimental Protocol. Ditags and PET-Technologies for Transcript Identification and Review Use of Ditags with Emphasis on Gene Identification. From Sequencing Output to CAGE Tags: A Chapter on the Extraction of CAGE Tags from the Raw Output of the Sequencing Machine. Using CAGE Data for Quantitative Expression. Compare CAGE Expression to Microarrays and qPCR. Identification of Isoforms. Promoter Analysis Databases. Construction and Tutorial of the CAGE Analysis Viewer and Genome Viewer. Transcriptional Network Analysis Using CAGE. Biological Examples of Alternative TSS/Promoters. Challenges in CAGE Analysis.

Szczegóły: Cap-Analysis Gene Expression - P Carninci

Tytuł: Cap-Analysis Gene Expression
Autor: P Carninci
Producent: World Scientific Publishing Co Ltd
ISBN: 9789814241342
Rok produkcji: 2008
Ilość stron: 250
Oprawa: Twarda
Waga: 0.57 kg

Recenzje: Cap-Analysis Gene Expression - P Carninci